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Detecting the Silent Killer: Clinical Diagnosis of Diabetes

Over 380 million people worldwide are afflicted by diabetes mellitus, a chronic disease that leads to high blood sugar. Due to genetic predisposition and high-calorie, low-activity lifestyles, that number continues to grow. Without early detection and treatment of diabetes, severe medical complications can occur. In this simulation, students will diagnose diabetes in three patients using the urine glucose test and Enzyme-linked Immunosorbent Assay (ELISA).

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In Search of the Kissing Disease

Infectious mononucleosis is commonly known as the "kissing disease". The causative agent is Epstein-Barr virus (EBV) which can be transmitted through saliva during kissing. In this experiment, students search for the presence of EBV using the ELISA reaction to detect specific viral proteins.

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Detection of Genetically Modified Organisms

For centuries, humans have used selective breeding and conventional hybridization to produce desirable qualities and to increase crop yields. Today, scientists use genetic engineering to directly manipulate the DNA, quickly producing these desirable traits. In this experiment, students will use agarose gel electrophoresis to explore the molecular methods used by scientists to identify genetically modified organisms. No thermal cycler is required. Students are also encouraged to explore the controversy surrounding the use of genetically modified organisms.

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Whose Fingerprints Were Left Behind?

After a crime has been committed, the evidence left behind can identify a potential culprit, although a single piece of evidence is not usually enough to convict someone. Even in this age of DNA, fingerprints and blood stains are still important at helping to identify a criminal. In this experiment your students will learn to detect and analyze fingerprints and then use these techniques to solve a classroom crime.

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