TRI Reagent® RT is an upgraded version of the single-step method of RNA isolation. TRI Reagent® RT is based on the phase separation method using phenol and guanidine thiocyanate. This patent-pending reagent substantially improves the quality of isolated RNA. Unlike the previous single-step methods, TRI Reagent® RT isolates RNA that is free of DNA contamination. The purity of RNA was tested by a 35-cycle PCR. Thus, no DNase treatment is necessary to use the isolated RNA in RT-PCR. Also, the isolated RNA solubilizes more quickly and easily as compared with the older single-step methods.

TRI Reagent® RT provides a reliable, cost-effective and efficient method of RNA isolation. It is a comprehensive reagent available for analysis of gene expression. TRI Reagent® RT isolates RNA from a wide variety of samples of human, animal, plant, yeast, bacterial and viral origin. TRI Reagent® RT also can be used to simultaneously isolate RNA, DNA and proteins from the same sample. The RNA isolation is completed in less than 1 hour. In addition to total RNA, TRI Reagent® RT can be used to isolate high and low molecular weight RNA as separate fractions.